Sensitive, soluble chromogenic substrates for HIV-1 proteinase.
نویسندگان
چکیده
منابع مشابه
Macromolecular chromogenic substrates for measuring proteinase activity.
BACKGROUND Proteinase activities are often measured using chromogenic substrates that are much smaller than physiological substrates. METHODS The hydrodynamic size of macromolecular substrates (macrosubstrates) prepared by linking small chromogenic substrates to polyethylene glycol was determined by gel filtration. Efficiency of macrosubstrate cleavage by proteinases and alpha(2)-macroglobuli...
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Hydrosoluble macromolecular fluorogenic substrates specific for the human immunodeficiency virus 1 (HIV-1) proteinase have been prepared. The fluoresceinyl peptide Ftc-epsilon-Ahx-Ser-Phe-Asn-Phe-Pro-Gln-Ile-Thr-(Gly)n, corresponding to the first cleavage site of HIV-1 gag-pol native precursor was linked to a water-soluble neutral (Lys)n derivative. The epsilon-aminohexanoyl residue (epsilon-Ah...
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Various ester and thioester derivatives of hippuric acid have been prepared which were substrates of both beta-lactamases and DD-peptidases. The thioesters were more rapidly hydrolysed by nearly all the enzymes. Surprisingly, the enzymes acted rather efficiently on substrates which did not contain any chiral centre.
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Spectrophotometric heparin assays which are based on the catalytic effect of heparin on either the inactivation of thrombin or that of factor Xa by antithrombin III, were adapted for use in a laboratory batch analyzer. Optimal conditions were determined for assays using the chromogenic substrates Chromozym-Th and S-2238 with thrombin, and S-2222 with factor Xa. Inactivation of the clotting enzy...
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Blood plasma contains a number of proteins capable of inhibiting the activation of plasminogen and the action of plasmin. Except for urokinase, the nature and action of plasminogen inhibitors are not yet fully understood. Inhibitors of plasmin, on the other hand, are fairly well defined and their clinical significance recognised. Although there are a number of plasmin inhibitors in plasma-for e...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1990
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)38989-6